Composite
Part:BBa_K1428001:Design
Designed by: Lai Hei Ming Group: iGEM14_Hong_Kong_HKU (2014-11-02)
Gene cluster for Expressing a purifiable Eut Bacterial Microcompartment
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 484
Illegal NotI site found at 1394 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 697
Illegal BglII site found at 1607
Illegal XhoI site found at 492
Illegal XhoI site found at 1402 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1321
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 909
Design Notes
Careful considerations about the linker peptide between the His6-tag and EutS must be considered to avoid (1) out-frame fusion and (2) linker peptides which are hydrophobic and inflexible.
Source
BBa_K311004, pET28a