Composite

Part:BBa_K1428001:Design

Designed by: Lai Hei Ming   Group: iGEM14_Hong_Kong_HKU   (2014-11-02)


Gene cluster for Expressing a purifiable Eut Bacterial Microcompartment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 484
    Illegal NotI site found at 1394
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 697
    Illegal BglII site found at 1607
    Illegal XhoI site found at 492
    Illegal XhoI site found at 1402
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1321
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 909


Design Notes

Careful considerations about the linker peptide between the His6-tag and EutS must be considered to avoid (1) out-frame fusion and (2) linker peptides which are hydrophobic and inflexible.


Source

BBa_K311004, pET28a

References